Information-enriched characterization of cell populations. Morphological information and facts from pictures can increase into the richness of flow cytometry knowledge. For instance, the determine reveals an normally typical apoptosis assay utilizing Annexin V and PI, adding mobile imaging to characterize cells in each inhabitants to reveal morphologically distinctive characteristics. These insights couldn't have been received from multiplex staining alone.
No matter if you’re a rookie or an authority, check out our assets to progress your exploration and know-how in flow cytometry.
Merge acoustic focusing technological innovation with high-parameter analysis for versatile spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing that has a high-speed brightfield digicam for simultaneous significant throughput flow cytometry and significant resolution brightfield imaging.
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Right here, a seasoned person has gated singlets confidently. Immediately after assessing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains more than four% aggregates.
Optimizing gating strategy. Even robust handbook singlet gating is mistake-susceptible and continues to be a subjective determination issue in Just about all flow cytometry assays. Imaging may be used to substantiate and modify gates to include only one cells of desire.
Our experts will style and design panels for you as over and over you will need—cost-free, no products acquire needed.
The Attune NxT cytometer is the reputable workhorse instrument your lab can depend on for dependable and reliable general performance. The Attune CytPix model features a substantial-speed brightfield digital camera and AI-pushed image Assessment software, with recent updates which includes equipment Studying instruments and an intuitive person interface, enabling Improved information precision, precision, and more quickly insights into sample biology.
Consumers can check out occasion images in serious-time to substantiate sample excellent, enhance protocols, and history the morphology of each cell inhabitants discovered for Examination. Imaging capture also https://museumcollection34444.wikienlightenment.com/7569749/flow_cytometer_a_revolutionary_tool_for_cell_analysis benefits from acoustic focusing, which helps you to placement the cells so that a sharp, centered image is attained.
Seem waves allow specific mobile alignment, leading to Outstanding details good quality and ~10X more rapidly sample flow rates.
Software Take note: Automatic image Examination decreases consumer-to-person variability in flow cytometry gating procedures
Designed-in dimensionality reduction instruments improved resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify envisioned inhabitants resolution in lysed whole human blood. Person can view functions from Each individual cluster for visual confirmation of mobile phenotypes.
Versatile payment plan from Agilent Economical Methods permit you to get the important instrumentation you would like for fast task, or to expand your lab.
At the guts of every Attune Flow Cytometer lies our innovative acoustic concentrating technological innovation, which makes use of audio waves to meticulously align cells inside the flow cell. This impressive solution surpasses conventional hydrodynamic solutions, resulting in more rapidly processing speeds, Remarkable information high quality, and elevated sample throughput.
As the mobile line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and had been capable of doc the microbial infection not less than five days before.
Agilent Benefit Guarantee assures you a minimum of a decade of instrument use within the date you purchase. Or else, we will credit rating you Along with the residual worth of the process toward an upgraded product.
What seemed to be a homogeneous population is definitely a lot more various—an insight that ought to be considered when interpreting benefits.